Back to Blog
Ffa in modtools7/3/2023 ![]() Fresh 50 mM FFA stock solutions were prepared in 90% ethanol. Mixtures 1a-2b were composed of equal parts of the FFA compositions depicted in Table S1. The percentages of single FFAs for each composition were taken from 5, 8, 9, 10, 11, adapted and listed in Table S1. The FFA compositions contain FFAs (all from Sigma-Aldrich, Munich, Germany C22:1 from Larodan AB, Solna, Sweden) in different volumetric percentages mimicking various popular edible oils and butter. Rat pancreatic islets were isolated as described 7. Human EndoC-βH1 beta-cells (ENDOCELLS SARL, Paris, France) were cultured 6 and regularly tested for mycoplasma. In a last step, we tested the toxic effects of mixtures of the FFA compositions of four different edible oils in order to elucidate whether it is possible thereby to ameliorate the toxic potential of individual edible oil compositions. Since we did not include other constituents such as antioxidants, we were able to associate differences in toxicity to the different FFA species in these oils. We generated oil compositions mimicking the FFA profiles of these edible oils containing different long-chain saturated, monounsaturated, diunsaturated, polyunsaturated and medium-chain FFAs. Nowadays it has been replaced due to its toxicity by new rapeseed oil varieties with a very low erucic acid content 5. In addition, we analyzed the toxicity of the former traditional version of old rapeseed oil with its characteristically high content of the very long-chain erucic acid (C22:1). In the present study, we, therefore, compared the effects of different FFA profiles of thirteen popular edible plant oils and butter on human EndoC-βH1 beta-cells and of selected popular oils on isolated rat islets. Since individuals consume oils composed of various saturated and unsaturated FFAs, FFA mixtures represent the typical lipid component of the daily diet. An excessive fat intake may have a major negative impact on beta-cell function. In general, saturated FFAs are thought to be harmful due to lipotoxicity, while unsaturated FFAs are considered to be beneficial 4. On the other hand, coconut oil, rich in medium-chain FFAs, has been promoted for positive effects on health 1 although clinical trials could not provide solid evidence for this assumption 2. A prominent example is olive oil, containing mostly the monounsaturated free fatty acid (FFA) oleic acid, which is thought to have beneficial effects on cardiovascular health and the metabolic state in T2DM 3. ![]() ![]() Numerous studies have examined the effects of dietary oil consumptions on human health however, with conflicting results 1, 2, 3. The toxicity of FFAs to human EndoC-βH1 beta-cells was dampened in mixtures of FFA compositions with a significant content of medium-chain FFAs, but not with a significant proportion of unsaturated FFAs.ĭietary habits can have a major impact on health and may cause obesity and type 2 diabetes mellitus (T2DM). A composition with 50% of the very long-chain unsaturated FFA erucic acid caused high toxicity with concomitant peroxisomal H 2O 2 production. On the other hand compositions with high content of medium-chain FFAs revealed no toxicity. Compositions with prominent content of saturated as well as unsaturated long-chain FFAs showed moderate but significant toxicity both in human EndoC-βH1 beta-cells and rat islets, however, without further measurable metabolic impairments. Human EndoC-βH1 beta-cells were incubated with different oil compositions and the toxicity, lipid droplet formation, ER-stress, and H 2O 2 production were analyzed. For this purpose, we made compositions consisting exclusively of various FFAs in different volumetric percentages mimicking these oils and additionally mixtures of these compositions. To investigate this issue, we examined the toxicity of free fatty acid (FFA) compositions mirroring the FFA profiles of various popular edible oils in human EndoC-βH1 beta-cells and in rat islets. An inappropriate diet, particularly excessive consumption of dietary fats and oils, may have a major negative impact on beta-cell function and cause type 2 diabetes mellitus. ![]()
0 Comments
Read More
Leave a Reply. |